Tagging Salt Tolerant Gene Using PCR Markers in Soybean
摘要
The purpose of this study was to screen and identify PCR markers associated with tolerant gene in soybean( Glycine soja L. ) so that tolerance can be identified efficiently and accurately. Between these tolerant and sensitivity to and three crosses were tested in this experiment. By BSA method, two codominant PCR markers were identified through the tolerant (sensitive) cuitivars bulks and the tolerant (sensitive) individual bulks of a F2 population. There was a 600bp band in the sensitive individuals and a 700bp band or two 700bp/600bp bands in the tolerant individuals. The markers were closely linked with tolerant/sensitive alleles. Moreover the markers were tested in the other two F2 populations from salt tolerant cultivar × sensitive cuitivar and confirmed by 12 tolerance cultivars and 13 sensitive cultivars with different genetic background. It indicated that the markers (700bp and 600bp) could be applied in tolerant identification of the soybean germplasm resources, and markers-assisted selection in tolerant breeding of soybean. The markers, its obtained method and application were patented for invention in 1998.