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Effect of Lanthanum（ III ） on Cytosolic Free Calcium in Isolated Rabbit Mature Osteoclasts

张金超、张天蓝、许善锦、王夔、于世凤、Mengsu Yang

2005Acta Scientiarum Naturalium Universitatis SunyatseniBiochemistry, Genetics and Molecular Biology被引 5

摘要

The effect of lanthanum(Ⅲ) (La3+) on cytosolic free calcium ([Ca2+]i) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La3+ does not alter basal [Ca2+]i levels and cell spread area at the concentration of 1.00×10-8 mol·L-1. However, La3+ at higher concentrations ( 1.00×10-5 and 1.00×10-7 mol·L-1) decreases [Ca2+]i levels and cell spread area, and greater decreases are observed for the higher concentrations of La3+. Since [Ca2+]i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La3+ inhibit bone resorption by decreasing [Ca2+]i in rabbit mature osteoclasts.

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