Membrane Microfiltration Fermentation of Glucose Oxidase with Cell Recycling
摘要
Membrane microfiltration fermentation (MMF) with cell recycling was successfully ap-piled to the production of glucose oxldase (GOD). A plate mlcrofiltmtion module was found suitable for such purpose. By feeding whole medium in MMF, the productivity of GOD was much higher than that by feeding glucose alone. With increasing dilution rate the enzynte productivity increased and average enzyme activity decreased, The enzyme productivity of MMF under D = 0.12h^-1 and 8.28h^-1 were 3871 and 3945 U-h^-1 respectively, which was about 3 times as that of hatch fermen-tation (BF) and the average enzyme activity was still as high as 37U·mL^-1 under D = 0.12h^-1. The relative efficiency of MMF applied to low yield strain was higher than that applied to high yield strain.