不同抗性小麦根与菲利普孢囊线虫（Heterodera filipjevi）互作的表型特征
摘要
Heterodera filipjevi is a newly identified pathogenic species of cereal cyst nematode that invades wheat ( Triticum aestivum L.) in central China. A two-year field test (2009–2011) conducted in Xuchang County, Henan Province, China, where H. filipjevi has severely damaged wheat for several years, demonstrated that the wheat-rye ( Secale cereale L.) chromosome substitution line 6R(6D) (carrying gene CreR for resistance to Heterodera spp.) was highly resistant (HR) to the nematode. The wheat cultivar Taikong 6 was moderately resistant (MR) and its parental cultivar Yumai 49 was highly susceptible (HS). Attraction of H. filipjevi juveniles to root tips of wheats differed in resistance to H. filipjevi was studied using Pluronic F-127 gel as a medium. Although root tips from wheat cultivars with different resistance were able to attract juveniles of nematode alone, the number of juveniles around the root tip from line 6R(6D) was obviously less than that from Taikong 6 (MR) and Yumai 49 (HS) when they were challenged together concurrently by H. filipjevi . The juveniles were capable to penetrate into wheat roots regardless of their resistance as revealed by acid fuschin-sodium hypochlorite staining of infected roots. However, significantly smaller number of nematodes in the roots of 6R(6D) and Taikong 6 were observed compared to those of Yumai 49 at later stage of wheat- H. filipjevi interaction. These findings demonstrate that some of the H. filipjevi juveniles are not able to continue to reproduce themselves although they can penetrate the roots of resistant wheat cultivars 6R(6D) and Taikong 6. The results from this study provide experimental evidence on mechanism of host resistance to H. filipjevi .