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用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定

张晓勤薛大伟周伟辉邬飞波张国平

2011DOAJ (DOAJ: Directory of Open Access Journals)Agricultural and Biological Sciences被引 2

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摘要

The seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M<sub>2</sub> generation, biological and agronomic characters were examined, and the identified mutants were validated in M<sub>3</sub> generation. Totally 97 phenotype mutants, involved in seedling habits, maturity, leaf morphology, plant height, spike shape and spikelet fertility were obtained from 3 000 treated plants, with mutated frequency of 7.46%. The results indicate the feasibility of developing barley mutant population using EMS treatment and the discussion is done on forward genetics approach of gene function study using mutant population for barley genetic improvement.

引用本文(GB/T 7714)

张晓勤, 薛大伟, 周伟辉, 等. 用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定[J]. DOAJ (DOAJ: Directory of Open Access Journals), 2011.

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DOI:https://doi.org/10.3785/j.issn.1008-9209.2011.02.008

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