Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato
摘要
Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang wasconducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase (GUS) and neomycin phosphotransferase (NPT Ⅱ ) genes. The results indicated that embryogenicsuspension cultures precultured for 1 -3 d were suitable for the transformation. The optimal cocultivation timewas 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.
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ZHAIHong, LIUQing-chang. Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2003.
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