[Determination of salicin in extract of willow bark by high performance liquid chromatography].
摘要
An HPLC method for the determination of salicin in extract of willow bark is described. Chromatographic analysis was carried out on a Kromasil C18, 5 microns column(4.6 mm i.d. x 250 mm) with methanol-0.01 mol/L KH2PO4 buffer (pH 4.01) (15:85, volume ratio) as mobile phase. The detection wavelength was 265 nm. Salicin was extracted from samples with methanol-water(50:50, volume ratio), and centrifuged. Ten microL of supernatant were injected. The average recoveries were from 96.1% to 101.2% (n = 5), and the relative standard deviation (RSD) was 1.43%. The method is simple, rapid and accurate.
引用本文（GB/T 7714）
L S Li, Weidong Huang, Qing He, 等. [Determination of salicin in extract of willow bark by high performance liquid chromatography].[J]. PubMed, 2001.
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