A New Voltammetric Enzyme Immunoassay System for the Detection of Alkaline Phosphatase
摘要
A new voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP) as the substrate for alkaline phosphatase (ALP). PNPP is enzymatically hydrolyzed and the product p-nitrophenol (PNP) is detected by differential pulse voltammetry (DPV), which can be oxidized at +1.02 V (vs. Ag/AgCl) on bare glass carbon electrode (GCE). The conditions for enzymatic reaction and electrochemical detection were studied. According to this method, ALP can be detected with a detection limit of 2.8′102 mU/L and a linear range of 4.0′102 ~ 1.0′106 mU/L.
引用本文（GB/T 7714）
KuiJIAO, Wei Sun. A New Voltammetric Enzyme Immunoassay System for the Detection of Alkaline Phosphatase[J]. 中国化学快报：英文版, 2002.
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