Identification and expression analysis of spinach AMT gene family
摘要
Ammonium nitrogen transporters (AMT) are responsible for the absorption and transportation of ammonium nitrogen, and also play important roles in plant growth and development. In this paper, the <i>SoAMT</i> gene family members were identified in the spinach genome, and their expression patterns were analyzed. Results showed that there were six SoAMT members in spinach genome, including five AMT1 and one AMT2, which were mainly distributed on 1, 4, 5, and 6 chromosomes. The length of their coding regions ranged from 1 443 to 1 506 bp. All SoAMT members had the unique conserved domains and conserved motifs of the AMT gene family, and 9-11 trans-membrane domains. Their promoters contained many elements about jasmonic acid response, anaerobic stress response, and so on. The <i>SoAMT</i> genes were expressed in roots, leaf blades, and petioles. Most <i>SoAMT</i>1 genes were induced by nitrogen deficiency, nitrate nitrogen, or ammonium nitrogen, while <i>SoAMT</i>2 expression was inhibited by ammonium nitrogen. The obviously different nitrogen response patterns between two types of subfamily members might be related to their different roles in response to nitrogen changes.