Isolation, screening and identification of superior yeasts from grape and orchard soil in Inner Mongolia
摘要
In this study, using different varieties of grapes and orchard soils collected from different regions of Inner Mongolia as samples, the yeasts in the samples were isolated and purified by traditional culture separation method, the primary screening was carried out by WL nutrient agar medium combined with morphological observation, the superior yeasts were screened by tolerance analysis and alcohol production ability determination, and their growth ability was analyzed. The superior yeasts were identified by physiological and biochemical tests and molecular biology techniques. The results showed that a total of 104 suspected yeast strains were isolated and purified, 42 yeast strains were preliminarily screened by WL medium, and 16 superior yeast strains with strong tolerance and high alcohol production capacity were obtained by re-screening, numbered as TJN1-TJN3, WHYN1, WHYN3, BHN5, EMN2, WLT1, XHN2, DB1-DB3, DR3, DJN3, XH3, DYT2, respectively. The 16 superior yeast strains could tolerate an environment with alcohol content 13%vol, pH 2.5, and glucose content 40%, and had a strong ability to produce alcohol. The 16 superior yeast strains were identified as Saccharomyces cerevisiae. This experiments laid a foundation for the screening of strains for local wine brewing in Inner Mongolia.