Effects of Different Foxtail Millet Intake on Gut Microbiota and Short Chain Fatty Acids in Mice(不同摄入量小米对小鼠肠道菌群和短链脂肪酸的影响)
摘要
Coarse cereals intake can reduce the incidence of some chronic metabolic diseases. However, there is no uniform standard for coarse cereals intake, and unknown whether high coarse cereals intakes have adverse effect on health remains. In this study, 3-week-old C57BL/6J mice were intervened by adding different ratios of foxtail millet in feed, including 20%, 40%, 60%, and 80% additions for 12 weeks. Using the automatic biochemical analyzer, high-throughput sequencing of 16S rRNA gene, gas chromatography mass spectrometry, the effects of different ratios of foxtail millet on lipid blood levels, gut microbiota and short-chain fatty acids in mice were analyzed. The results showed that the feed with 80% supplementation foxtail millet significantly increased the total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, propionic acid, butyric acid, isobutyric acid and valeric acid. In addition, foxtail millet intake increased the abundance of Bacteroidota and Muribaculaceae, decreased the abundance of Firmicutes, Actinobacteriota, Lactobacillaceae and Bifidobacterium. The gut microbiota composition in mice varied with different levels of foxtail millet. The microbiota of mice fed with the supplementation of 20% were enriched in Alistipes, Parabacteroides, Enterorhabdus. However, the abundances of Faecalibaculum, Blautia and Roseburia were reduced for the mice fed with the supplementation of 80%. The results showed that, 20% intake of foxtail millet was sufficient to regulate gut microbiota, while 80% intake led to abnormally elevated lipid levels in blood, reduced the diversity and evenness of gut microbiota, and decreased the abundance of probiotics. Therefore, coarse cereals' nutritional value should be rational considered and excessive intake patterns for coarse cereals should be avoided.(摄入一定量杂粮可降低一些慢性代谢疾病的发病率,但目前杂粮摄入量没有统一的标准,尚不清楚杂粮摄入过多是否会对健康产生不良影响。以小米添加量为20%、40%、60%、80%的饲料喂养3周龄C57BL/6J小鼠,持续12周,采用自动血生化分析仪、16S rRNA高通量基因测序、气相色谱-质谱联用仪分