Establishment of Continuous Multi-Stage Countercurrent Water Extraction Process of Tea Saponin from Camellia Meal(油茶粕中茶皂素连续多级逆流水提工艺的建立)
摘要
In order to improve the economic value of camellia meal and make full use of the camellia meal resources, tea saponin was extracted from camellia meal by continuous multi-stage countercurrent extraction process using hot water, and the parameters of single-stage extraction and continuous multi-stage countercurrent extraction process were optimized. The results showed that the best conditions for single-stage extraction were as follow:extraction time 85 min, extraction temperature 80℃, material-liquid ratio 1∶16g/mL, and the most suitable extraction size of camellia meal 60-100mesh. On the basis of single-stage extraction, the optimal number of extraction stages for continuous multi-stage countercurrent extraction was determined to be 3 stages, and the optimum extraction time for each stage was 20min. Under those conditions,the content of tea saponin in the extract was 33.14mg/mL, which was 123% higher than that of single-stage extraction. Extraction time was shortened 29.41% and extraction efficiency was improved at the same time. In this study, an aqueous solution combined with continuous multi-stage countercurrent extraction was used to obtain tea saponin with a higher mass concentration, and the extraction time was shortened. The extraction process was green and environmentally friendly, and industrial production was easier to achieve.(为提高油茶粕经济价值,充分利用油茶粕资源,以油茶粕为原料,采用热水连续多级逆流提取油茶粕中的茶皂素,优化了单级提取和连续多级逆流提取工艺参数。结果表明:单级提取的优化条件为提取时间85min,提取温度80℃,料液比1∶16g/mL,油茶粕提取粒度60~100目。在单级提取基础上,确定连续多级逆流提取的优化提取级数为3级,每级优化的提取时间为20min,此时得到的提取液中茶皂素的质量浓度为33.14mg/mL,比单级提取质量浓度提高了1.23倍,提取时间缩短了29.41%,提高了提取效率。 水溶液结合连续多级逆流提取,获得了质量浓度较高的茶皂素,并且缩短了提取时间,提取流程绿色环保,更易实现工业化生产。)