Screening and Evaluating of Saccharopolyspora with Biogenic Amines Degrading Ability in Huangjiu(黄酒中降生物胺糖多孢菌的筛选及评价)
摘要
In order to obtain the strains with activity of reducing the content of biogenic amine in Huangjiu, Saccharopolyspora strains in the fermentation process of Huangjiu was isolated and identified, and its biogenic amine degradation ability was explored. In this study, 37 Saccharopolyspora strains were isolated and identified from wheat Qu and fermented mash of Huangjiu. Using biogenic amine production and biogenic amine degrading ability as evaluation indexes, two Saccharopolyspora strains (F1902 and F2014) with low biogenic amine production and strong biogenic amine degrading ability were obtained. According to the identification of molecular biology, physiological and biochemical indicators, the two strains were identified as Saccharopolyspora hirsuta and Saccharopolyspora rosea, respectively. The two strains were fermented in the medium containing biogenic amines for 5 days at 37℃. The total biogenic amines degradation rates of the two strains were 76.41%±0.82% and 74.62%±0.84%, respectively. The ability of the two strains to degrade biogenic amines under different environmental conditions was further studied, the results showed that temperature, pH value and ethanol concentration all affected the growth of Saccharopolyspora and the ability of reducing biogenic amines. The optimum temperature and pH value for S. hirsuta F1902 and S. rosea F2014 to degrade biogenic amines was 30℃, and 7, respectively. And a certain biogenic amine degradation ability was still exerted in the presence of volume fraction 7% ethanol. The research might provide a theoretical basis for the control of biogenic amine content during the brewing process of Huangjiu by Saccharopolyspora and the development and utilization of Huangjiu as biological resources.(为获得可降低黄酒中生物胺含量的菌株,研究对黄酒发酵过程中的糖多孢菌进行了分离鉴定,并对其生物胺降解能力进行了探究。研究从黄酒麦曲和发酵醪中共分离鉴定得37株糖多孢菌,以菌株产生物胺和降生物胺能力为评价指标,筛选获得2株低产生物胺且具有较强降生物胺能力的糖多孢菌(F1902和F2014)。经对菌株的分子生物学、生理生化指标进行鉴定,2株菌分别为披发糖多孢菌(Saccharopolyspora hirsuta)和玫瑰糖多孢菌(Saccharopolyspora rosea)。将