Effect of Tropomyosin of Macrobrachium nipponense on Mouse Macrophage Polarization Based on Transcriptomic Analysis(基于转录组学分析日本沼虾原肌球蛋白对小鼠巨噬细胞极化的影响)
摘要
To study the effect of tropomyosin of Macrobrachium nipponense on macrophage polarization, the differential analysis of gene expression during tropomyosin-induced macrophage polarization was analyzed by transcriptomic analysis. The results showed that compared with the phosphate buffer saline group (PBS group), 69 genes were up-regulated and 154 genes were down-regulated in the tropomyosin-induced group (TM group), and 180 pathways were enriched. In the lipopolysaccharide and IFN-γ combined-induced M1 macrophage group(LPSIFN group), 1346 genes were up-regulated, 1360 genes were down-regulated, and 308 pathways were enriched. Compared with the IL-4-induced M2 macrophage group(IL4 group), 455 genes were up-regulated in the TM group, 446 genes were down-regulated and enriched to 269 pathways. According to the KEGG results, five signaling pathways that may be involved in macrophage polarization were selected, including NOD-like receptor signaling pathway, Jak-STAT signaling pathway, NF-κB signaling pathway, Toll-like receptor signaling pathway and PI3K/Akt signaling pathway. The expression of NOD2, TLR2, AKT3, NLRP3, Caspase-1, CD14, Lat, Myd88, NFKBIA and STAT1 genes in these five pathways were verified, and the expression levels of NOD2, TLR2, NLRP3, and STAT1 in the TM group were increased significantly. The NOD-like receptor signaling pathway, Jak-STAT signaling pathway, and Toll-like receptor signaling pathway related to these four genes were involved in the process of tropomyosin-induced macrophage polarization. The purpose of this study was to provide a theoretical basis for the further analysis of the relationship between macrophage polarization and food allergic relationships.(为研究日本沼虾原肌球蛋白对巨噬细胞极化的影响,运用转录组学分析原肌球蛋白诱导的巨噬细胞极化过程中基因表达的差异性。结果表明:与磷酸盐缓冲溶液组(PBS组)相比,原肌球蛋白诱导组(TM组)有69个基因表达上调,154个基因表达下调,富集到180条通路；TM组相较于脂多糖和IFN-γ联合诱导M1型巨噬细胞组(LPSIFN组)有1346个基因表达上调,1360个基因下调,富集到308条通路；TM组与IL-4诱导M2型巨噬细胞组(IL4组)相比,有455个基因上调,446个基因下调,富集到269条通路。根据KEGG结果选择5条可能涉及巨噬细胞极化的信号通路,包括NOD样受体信号通