Effects of Anthocyanins from Black Rice on Spatial Learning and Memory in Mice Fed with High Fat Diet(黑米花青素对高脂膳食小鼠的空间学习记忆能力的影响)
摘要
To investigate the effects of anthocyanins from black rice on spatial learning and memory in C57BL/6 mice fed with high fat diet. 48 C57BL/6 mice were randomly divided into 4 groups:normal chow diet (NCD) group, high fat diet (15%) (HFD) group, high fat diet plus 40mg/kg anthocyanins (LBA) group, and high fat diet plus 200mg/kg anthocyanins (HBA)group. After 12 weeks, the cognitive components of behavior were evaluated by Morris water maze test. Serum total cholesterol and triglyceride, oxidative stress index, monoamines content of hippocampus in mice were determined. The expressions of inflammatory cytokines in hippocampus were determined by real-time PCR. The results indicated that spatial learning and memory ability, activity of SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase) in hippocampus of mice in HFD group were significantly reduced. The serum total cholesterol, triglyceride, MDA (malondialdehyde), and inflammatory factors TNF-α, COX-2, and IL-1β mRNA were dramatically increased. However, supplementation of anthocyanins from black rice significantly elevated the spatial learning and memory ability of mice, the oxidative status in hippocampus was dramatically improved, and the mRNA levels of inflammatory factors were significantly reduced. In summary, HFD led to chronic oxidative stress, impaired the cognitive ability such as hippocampus-dependent spatial learning and memory. Anthocyanins from black rice, could improve the oxidative stress, increase the activity of SOD and GSH-Px, reduce the production of MDA and the mRNA levels of inflammation factors, and finally improve the learning and memory ability in mice.(探讨高脂膳食对C57BL/6小鼠以及黑米花青素对高脂膳食小鼠的空间学习记忆能力的影响。将48只小鼠随机分成对照组(正常膳食),高脂对照组(膳食中含w=15%猪油),高脂低剂量黑米花青素组(膳食中含w=15%猪油、w=40mg/kg黑米花青素)和高脂高剂量黑米花青素组(膳食中含w=15%猪油、w=200mg/kg黑米花青素)。12周后通过Morris水迷宫实验评价小鼠的空间学习记忆能力,检测小鼠血清总胆固醇和总甘油三酯,小鼠海马体中氧化应激指标、单胺类神经递质含量以及炎症相关因子基因的mRNA表达水平。研究发现,高脂膳食的小鼠空间学习记忆能力显著下降(P<0.05),血清总胆固醇和总甘油三酯含量显著增加(P<0.05),海马组织中的SOD(superoxide dis