Inhibitory Effect on α-Glucosidase Activity of Polyphenol from Red-skin of Lotus Seed(莲子红衣多酚对α-葡萄糖苷酶的抑制作用)
摘要
The aim of the research was to discuss the inhibitory effect of polyphenol from lotus seed red-skin on α-glucosidase activity. It was designed to measure the α-glucosidase activity in different conditions using spectrophotometry. With the inhibitory effect on α-glucosidase activity as the index, the inhibitory conditions were optimized by the L<sub>9</sub> (3<sup>4</sup>) orthogonal optimization method. The results revealed that the optimal inhibition rate was 67.99%±1.79% at pH 6.8,1.50g/L of polyphenol concentration and 20min of reaction time. In addition, polyphenol was an anti-competitive inhibition type of inhibitor on α-glucosidase, and its inhibition constant was 0.24g/L.(采用分光光度法测定了不同条件下莲子红衣多酚对α-葡萄糖苷酶活性抑制作用的影响,在单因素实验基础上运用L<sub>9</sub>(3<sup>4</sup>)正交法得到莲子红衣多酚对α-葡萄糖苷酶活性抑制作用的优化条件。研究结果显示,在pH 值为6.8,质量浓度为1.50g/L,反应时间为20min时,莲子红衣多酚对α-葡萄糖苷酶活性的抑制率最高,可达67.99%±1.79%。莲子红衣多酚对α-葡萄糖苷酶的抑制作用类型为反竞争性抑制,抑制常数为0.24g/L。)