Expression of a Carrot Antifreeze Protein Gene in Escherichia coli
摘要
The recombinant expression vectorpET43. lb-AFP, which contains full encoding region of a carrot 36 kD antifreeze protein (AFP) gene was constructed. The recombinant was transformed into expression host carrying T7 RNA polymerase gene (DE3 lysogen) and induced by 1 mmol. L-1 IPTG (isopropyl-β-D-thiogalactoside) to express 110 kD polypeptide of AFP fusion protein.The analysis of product solubility revealed that pET43. 1b-AFP was predominately soluble, and the expressed amount reached the maximum after the IPTG treatment for 3 h.
引用本文（GB/T 7714）
Ma -, Xinyu, Shen, 等. Expression of a Carrot Antifreeze Protein Gene in Escherichia coli[J]. 中国林学：英文版, 2003.
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