cDNA Fragment Cloning of L-Galactono-1,4-Lactone Dehydrogenase andIt＇s Expression in Different Organs of R. roxburghii Tratt
摘要
A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequence analysis showed 79-92% identity in nucleotide sequence and 75-87%identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc.Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root respectively. Furthermore, this expression mode was highly correlated with AsA levels.
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ANHua-ming, CHENLi-geng, FANWei-guo. cDNA Fragment Cloning of L-Galactono-1,4-Lactone Dehydrogenase andIt＇s Expression in Different Organs of R. roxburghii Tratt[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2004.
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