[Effect of electroacupuncture on cannabinoid-related receptor CB2R and TRPV1 and spinal microglial polarization in rats with primary dysmenorrhea].
摘要
OBJECTIVES: To observe the effect of electroacupuncture (EA) on the expression of cannabinoid receptor type 2 (CB2R), atypical cannabinoid receptor transient receptor potential vanilloid 1 (TRPV1), and spinal microglial polarization in rats with primary dysmenorrhea (PDM), so as to explore the underlying mechanism of EA alleviating PDM pain hyperalgesia. METHODS: =10 rats per group). The PDM model was established by subcutaneous injection of estradiol diphenhydrate combined with intraperitoneal injection of oxytocin. Rats in the EA group were treated with EA (50 Hz) at "Guanyuan" (CV4) and bilateral "Sanyinjiao" (SP6) for 20 min, once a day for 10 consecutive days. The number of writhing, writhing score, and writhing latency were observed. The uterine histopathological changes were observed by H.E. staining. The contents of prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2) in serum and interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in spinal cord were detected by ELISA. The protein expressions of CB2R and TRPV1 in spinal cord and uterus, and inducible nitric oxide synthase (iNOS) and arginase-1 (Arg1) in spinal cord were detected by Western blot. RESULTS: <0.001) in the EA group. H.E. staining showed degeneration, swelling, and necrosis of endometrial epithelial cells, dilation of glands, stromal hyperemia, and granulocyte infiltration in the model group, which were relatively milder in the EA group. CONCLUSIONS: EA can alleviate uterine inflammatory response, pathological damage and pain sensitization in PDM rats, which may be related to activating peripheral and central CB2R expression, inhibiting TRPV1 expression, promoting spinal cord microglia polarization from M1 to M2, thus reducing neuroinflammation.