[Establishment and preliminary evaluation of recombinase-aided isothermal nucleic acid amplification combined with nanopore sequencing for identification of <i>Plasmodium</i> species].
摘要
OBJECTIVE: , and to prelimi-narily assess its detection performance. METHODS: DNA samples, and the 49 samples with the best amplification effect were selected for nanopore sequencing. The species identification of 49 dried blood spot samples from malaria patients was compared by RT-qPCR assay, nPCR assay and RAA-nanopore sequencing, and the sensitivity, specificity and accuracy of RT-qPCR assay and RAA-nanopore sequencing were evaluated with nPCR identification as the gold standard. RESULTS: mixed infection in one sample. If nPCR assay served as the gold standard, the sensitivity, specificity and accuracy of RAA-nanopore sequencing were 92.00%, 97.33% and 96.00% for species identification of malaria parasites, which were higher than those (88.24%, 97.32%, and 95.00%) of the RT-qPCR assay. CONCLUSIONS: species, which may serve as a supplementary approach to conventional techniques for detection of malaria parasites.