[Total Flavonoids from Arachniodes exilis Enhance Osteogenic Capacity of Mesenchymal Stem Cells via BMP Signaling].
摘要
Objective: To investigate the effects and its molecular mechanism of the total flavonoids from Arachniodes exilis( TFA)on the osteogenic differentiation of bone marrow mesenchymal stem cells( BMSCs). Methods: Alizarin red staining and alkaline phosphatase( ALP) activity were used to evaluate the osteogenic differentiation capacity of BMSCs; RT-PCR was used to assess the transcription level of collagen type Ⅰ α1( COL1A1),osteocalcin( OCN) and osteopotin( OPN),BMP2,BMP4,Runx2,Osterix; the expression of bone morphogenetic protein( BMP) signaling related proteins( BMP2,BMP4,Runx2) were detected by ELISA. Results: TFA enhanced osteogenic differentiation capacity of BMSCs significantly, demonstrated by increasing the formation of calcium nodules, ALP activity and gene expression of COL1A1,OCN and OPN, while those effects were dramatically attenuated by treatment with Noggin; TFA significantly upregulated the expression of BMP2,BMP4,Runx2 and Osterix. Conclusion: TFA promoted the osteogenic differentiation capacity of rat bone marrow mesenchymal stem cells through BMP signaling.