[Inhibition of TGF-β promotes functional recovery of spinal cord injury in mice by reducing fibronectin deposition].
摘要
OBJECTIVE: To investigate the effect of transforming growth factor (TGF-β) inhibition on functional recovery of spinal cord injury in mice. METHODS: =4). The mice in the treatment group were subjected to hemisection of the spinal cord and received intraperitoneal injection of TGF-β neutralizing antibody (1D11) 3 times a week (25 μL each time), and those in control group were injected with the vehicle antibody (13C4) following spinal cord hemisection. The sham-operated mice underwent sham operation to expose the spinal cord without hemisection. Four weeks later, the heart of the mice was perfused and 1-2 cm of the spinal cord spanning the injury site was harvested. Immunofluorescence staining of FSP1, fibronectin, and PGP9.5 was performed to assess fibroblast recruitment in the injury area, fibronectin deposition, and neurological recovery. For further verification of the results, we used a mouse model of spinal cord clamp injury to observe the survival of axons and distribution of astrocytes by detecting expressions of 5-HT and GFAP with immunofluorescence assay. RESULTS: < 0.05). CONCLUSION: Inhibiting TGF-β after spinal cord injury can reduce the recruitment of fibroblasts and fibronectin deposition to promote recovery of neurological function and repair of the injured spinal cord in mice.