The Efficient Transformation of Wheat in Planta by Agrobacterium tumefaciens
摘要
Transformation of wheat was performed by pipetting spikelets with Agrobacterium tumefaciens which contained expression vectors using Npt Ⅱ as reporter gene. Transformants were identified through kanamycin resistance, PCR and Southern blot. The results showed that transformation efficiency was within 2.0 to 3. 2 % in all tested varieties of wheat. Then the simple and efficient protocol of wheat transformation by Agrobacterium tumefaciens in planta was primarily established.
引用本文（GB/T 7714）
HEDao-yi, LIZhong-cun, WANGHong-gang. The Efficient Transformation of Wheat in Planta by Agrobacterium tumefaciens[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2003.
引文网络
本站仅收录题录与摘要供学习参考，全文版权归属出版方；如有侵权请联系我们删除。