一种基于多孔硅拉曼光谱分析的免疫传感器
摘要
Porous silicon (PS) suitable for optical detection of immunoreaction is fabricated.The structure of immunosensor is prepared by the following steps: oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody.When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A (spaA) of below 4.0 µg•ml -1 .The ultimate detection limit is 1.412 × 10 2 pg•ml -1 .Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity.Compared with the conventional enzyme-linked immuno sorbent assay (ELISA), this method is quick, inexpensive, and label-free.