rtificial Synthesis of TAT PTD-Tachyplesin Fusion Gene bv Overlao Extension PCR
摘要
This study aimed to investigate the synergism of the TAT PTD (protein transduction domain in HIV-1 transactivator of transcription protein) to antibacterial peptide tachyplesin from Tachpleus tridentatus.Treated with Pichia pastoris preferred codon optimization, using the cDNA sequence of tachyplesin mature peptide (54 aa) harboring TAT PTD sequence (11 aa) as reference template, six single-stranded oligonucleotides were designed, the sequences of restriction sites EcoR I and Xba I were introduced to the 5’ end of primers P1 and P6, respectively.TAT PTD +Tachyplesin fusion gene with a full length of 219 bp was artificially synthesized by overlap extension PCR, which laid the preliminary foundation for subsequent functional and synergism studies.