Molecular modeling of rice Rubisco
摘要
Molecular model of rice Rubisco has been constructed by molecular modeling method on a computer graphics workstation using graphics software TOM-FRODO and QUANTA/CHARMm,based on the three-dimensional structure of spinach Rubisco.The molecular modeling was carried out in the following procedure:amino-acid sequence substitution;manual adjustment of the initial model;and energy minimization.The three-dimensional structure of the quaternary complex Rubisco-CO2·Mg2+·CABP of rice Rubisco has also been modeled and optimized using the program package XPLOR.The main-chain structure of rice Rubisco does not show obvious difference from that of spinach Rubisco,and the side-chains are located in the most favorable positions and orientations.The active-site structures are basically the same for the two species.The mutant Leu335→Arg of rice Rubisco has been designed using the graphics software TURBO-FRODO and the program package XPLOR.Energy computation suggests that this mutation might increase the carboxylation