Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine
摘要
A strip reader based lateral flow immunoassay(LFIA)was established for the rapid and quantitative detection of ractopamine(RAC)in swine urine.The ratio of the optical densities(ODs)of the test line(AT)to that of the control line(AC)was used to effectively minimize interference among strips and sample variations.The linear range for the quantitative detection of RAC was0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration(IC50)of 0.59±0.06 ng/mL.The limit of detection(LOD)of the LFIA was 0.13 ng/mL.The intra-assay recovery rates were 92.97%,97.25%,and 107.41%,whereas the inter-assay rates were80.07%,108.17%,and 93.7%,respectively.
引用本文（GB/T 7714）
Ren Ren, Мэй, Ling, 等. Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine[J]. 生物医学与环境科学：英文版, 2014.
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