Carvacrol suppresses the expression of inflammatory marker genes in D-galactosamine-hepatotoxic rats
摘要
Objective:To unravel the mechanism of anti-inflammatory activity of carvacrol in D-galactosamine(D-GalN)-induced hepatotoxic rats.Methods:The mRNA and protein expression levels of tumor necrosis factor-alpha(TNF-α).interleukin-6[IL-6).inducible nitric oxide synthase(iNOS),cyelooxy genase-2(COX-2) and nuclear faclor kappa-B(NF-κB) were assayed by semi-quantitative reverse transcriptase polymerase chain reaction(RTPCR) and western blot analysis.Results:We found that the mRNA and protein expressions of TNF-α. IL-6,iNOS,COX-2 and NF-κB were significanlly up-regulated in D-galactosamine induced hepatotoxic rats and treatment with carvacrol significantly down-regulated the expressions of these genes showing the mechanism behind the anti-inflammatory activity of carvacrol. Conclusions:All above results reveal that the carvacrol well known anti-inflammatory activities in D-galactosamine induced hepatotoxic rats.