ISSR Polymorphism Analysis of Eight F1 Hybrids of Erianthus fulvus and Saccharum spp.
摘要
In this study, twelve primers were used for ISSR (inter simple sequence repeat) analysis of eight F1 hybrids of Erianthus fulvus and sugarcane (Saccharum spp.). The results showed that totally 133 bands were amplified, including 111 polymorphic bands, showing a polymorphic rate of 83.46%, which indicated that there are abundant genetic variations among F1 progenies derived from crossing between cultivated sugarcane and E. fulvus. The average genetic similarity coefficients between F1 progenies and female parent Yacheng 89-9, F1 progenies and male parent E. fulvus were 0.67 and 0.44, respectively. UPGMA clustering result of F1 progenies showed that all the progenies were clustered with female parent, suggesting that the genetic material of female parent was predominated in F1 progenies, which also revealed that the chromosome number of F1 progenies was not directly related to the clustering results based on ISSR analysis.