Genetic Transformation of Chinese Cabbage （Brassica rapa pekinensis） with Floral-dip Method

Yang、Gao、Xiangliang、Ren Ren、Songlin、؉ Ruan、Huoxu、Wang

2012农业生物技术：英文版Biochemistry, Genetics and Molecular Biology被引 1

摘要

[Objective] This study is aimed to transform gus gene into Chinese cabbage (Brassica rapa pekinensis) by floral-dip method. [Method] The Chinese cabbage was transformed by floral-dip method for the first time. Then the cabbage seeds were harvested and screened by hygromycin, and the plants with resistance were confirmed by histochemical GUS assay and PCR detection. [Result] The target gene was successfully integrated into the Chinese cabbage genome, and the transformation rate was 0.1%. [Conclusion] This study optimized the genetic transformation system for Chinese cabbage, and laid the foundation for improving the means of genetic transformation of Chinese cabbage.

引用本文（GB/T 7714）

Yang, Gao, Xiangliang, 等. Genetic Transformation of Chinese Cabbage （Brassica rapa pekinensis） with Floral-dip Method[J]. 农业生物技术：英文版, 2012.

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