The effect of beta-elemene on alpha-tubulin polymerization in human hepatoma HepG2 cells
摘要
Objective:To investigate the impact of beta-elemene injection on the growth and alpha-tubule of human hepatocarcinoma HepG2 cells.Methods:Cell proliferation was assessed by MTT assay.Cell cycle distribution was detected by flow cytometry(FCM).The mRNA expression of alpha-tubulin was measured by RT-PCR.Western blot analysis was used to determine protein expression of alpha-tubulin and the polymerization of tubulin.Results:Beta-elemene injection inhibited HepG2 cells proliferation in a dose-and time-dependent manner;FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase.RT-PCR and westernblot analysis showed that beta-elemene injection down-regulated alpha-tublin at both mRNA and protein levels,presenting a dose-dependent manner.Moreover,beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner.Conclusions:Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells and induce cell apoptosis,the mechanism might be partly related to the down-regulation of alpha-tubulin and inhibition of microtubular polymerization.