Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas
摘要
AIM:To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.sothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro.Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.RESULTS:Exogenous overexpression of mesothelin promoted cell proliferation,cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells.Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group.After an injection of adenovirusmesothelin,a significantly increased number of small islets appeared,and the expression of PCNA was decreased on day 7 and day 14 compared with the AdEGFP group.CONCLUSION:Mesothelin was able to promoteβcell proliferation in the remodeling stage of neonatal rats.Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.