Antibody Production for a Rapid Fluorescence Polarization Immunoassay of Estrone

Zhang、Xuân Xuân、Wang、Qiang Qiang、Yu、Zhong、Sergei、Eremin

2014生物医学与环境科学：英文版Engineering被引 3

摘要

Estrone has been identified as a potential endocrine-disrupting chemical(EDC)[1].Estrone is usually quantified by gas chromatography-mass spectrometry(GC-MS),GC-MS/MS,high performance liquid chromatography(HPLC),HPLCMS,and HPLC-MS/MS,etc.[2-3].Meanwhile,several immunoassays based on radioimmunoassay,enzyme linked immunosorbent assay(ELISA)or chemiluminescence immunoassay(CLIA)for determination of estrone in real samples have been developed[2,4].Although these methods are sensitive,they need multistage separation and are thus time-consuming and laborious.A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods(with separation)to homogeneous assays(without separation)[5].

引用本文（GB/T 7714）

Zhang, Xuân Xuân, Wang, 等. Antibody Production for a Rapid Fluorescence Polarization Immunoassay of Estrone[J]. 生物医学与环境科学：英文版, 2014.

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