In vitro clonal propagation of Achyranthes aspera L. and Achyranthes bidentata Blume using nodal explants
摘要
solutions for I min.After rinsing with sterile distilled water for 3-4 times,nodal segments were cut into smaller segments（1 cm）and used as the explants.The explants were placed horizontally as well as vertically on solid basal Murashige and Skoog（MS）medium supplemented with 3%sucrose,0.6%（w/v）agar（HiMedia,Mumbai）and different concentration and combination of 6-benzyl amino purine（BAP）,kinetin（Kin）,naphthalene acetic acid（NAA）and indole acetic acid（IAA）for direct regeneration.Results:Adventitious proliferation was obtained from A.aspera and A.bidentata nodal segments inoculated on MS basal medium with 3%sucrose and augmented with BAP and Kin with varied frequency.MS medium augmented with 3.0 mg/L of BAP showed the highest percentage（93.60±0.71）of shootlets formation for A.aspera and（94.70±0.53）percentages for A.bidentata.Maximum number of shoots/explants（10.60±0.36）for A.aspera and（9.50±0.56）for A.bidentata was observed in MS medium fortified with 5.0 mg/L of BAP.For A.aspera,maximum mean length（5.50±0.34）of shootlets was obtained in MS medium augmented with 3.0 mg/L of Kin and for A.bidentata（5.40±0.61）was observed in the very same concentration.The highest percentage,maximum number of rootlets/shootlet and mean length of rootlets were observed in 1/2 MS medium supplemented with 1.0 mg/L of 1BA.Seventy percentages of plants were successfully established in polycups.Sixty eight percentages of plants were well established in the green house condition.Sixty five percentages of plants were esta