Deletion of Salmonella enterica serovar typhimurium sipC gene

Maryam Maryam、Safarpour、Dehkordi、Abbas Abbas、Doosti、Asghar、Arshi Arshi

2015亚太热带生物医学杂志：英文版Biochemistry, Genetics and Molecular Biology被引 1

摘要

Objective:To construct a novel plasmid as Salmonella enterica serovar typhimurium(S.typhimurium)sip C gene knockouts candidate.Methods:In this research,50upstream and 30downstream regions of S.typhimurium sip C gene and kanamycin gene were PCR amplified.Each of these DNA fragment was cloned into p GEM T-easy vector.The construct was confirmed by PCR and restriction digest.Results:PCR amplified 320,206 and 835 bp DNA fragments were subcloned into p ET-32 vector resulting with a plasmid called p ET-32-sip C up-kan-sip C down.Conclusions:The new plasmid(p ET-32-sip C up-kan-sip C down)is useful for genetic engineering and for future manipulation of S.typhimurium sip C gene.

引用本文（GB/T 7714）

Maryam Maryam, Safarpour, Dehkordi, 等. Deletion of Salmonella enterica serovar typhimurium sipC gene[J]. 亚太热带生物医学杂志：英文版, 2015.

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