Cloning and prokaryotic expression of HGLP cDNA

Zhou、Haijun、Huang、Xiaowei Xiaowei、Yan、Li、Guangtao、Yin Yin

2001中国科学通报：英文版Biochemistry, Genetics and Molecular Biology被引 1

摘要

A novel cDNA, HGLP, which encodes a G-protein coupled receptor (GPCR) like protein, has been isolated and cloned. The coding region of the human HGLP predicts a 7-transmembrane region protein with motifs of rhodopsin-like GPCR superfamily. Northern blot analysis reveals a 3-kb transcript in various human tissues examined. The N- and C-terminal coding regions of HGLP, which are deduced as non-transmembrane regions, have been amplified by PCR and cloned into pET30a+ vector. Then the re-combinant proteins are highly expressed in E. coli.

引用本文（GB/T 7714）

Zhou, Haijun, Huang, 等. Cloning and prokaryotic expression of HGLP cDNA[J]. 中国科学通报：英文版, 2001.

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