上海地区HPV分型及高危型HPV负荷量与宫颈病变的关系
摘要
The epidemiological distribution of human papillomavirus (HPV) subtypes varies among different countries and also in different areas within the same country. HPV viral load is correlated with the grade of cervical intraepithelial lesion. Therefore, HPV subtype and HPV viral load testing have drawn increasing research attention. We investigated 515 cervical disease specimens collected from patients at the Shanghai Pudong New Area Gongli Hospital. We used guide hybrid gene chip technology (HybriMax) to conduct HPV genotyping and measured HPV viral load by RT-PCR. We then analyzed the correlations between HPV genotypes and grade of cervical disease, as well as HPV viral load and cervical disease. We detected 18 HPV subtypes in Shanghai. Six high-risk subtypes (HPV 16, 58, 33, 52, 31, 18) had a detection rate of over 5%, of which four subtypes (HPV 16, 58, 33, 52) were significantly correlated with the severity of cervical lesions. Spearman rank correlation analysis showed that HPV viral load and cervical lesion grade were positively correlated (<italic>r</italic>=0.318, <italic>P</italic>=0.000); this correlation was primarily as a result of HPV-16 and HPV-33. This study presents the prevalent HPV subtypes in Shanghai and analyzes HPV viral load and the relationship to cervical lesions. These results provide evidence for the clinical significance of HPV viral load in rapid screening for cervical lesions, and serves to inform for the development of a polyvalent vaccine for China.