Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells
摘要
Baicalin,a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi,has been shown to effectively inhibit cell apoptosis.Therefore,we assumed that baicalin would suppress colistin sulfate-induced neuronal apoptosis.PC12 cells exposed to colistin sulfate(62.5–500μg/mL)for 24 hours resulted in PC12 cell apoptosis.In addition,caspase-3 activity,lactate dehydrogenase level and free radical content increased in a dose-dependent manner.Subsequently,PC12 cells were pretreated with baicalin(25,50 and 100μg/mL),and exposed to 125μg/mL colistin sulfate.Cell morphology markedly changed,and cell viability increased.Moreover,caspase-3 activity,lactate dehydrogenase level and free radical content decreased.Results indicated that baicalin inhibited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury,and reducing caspase-3 activity and lactate dehydrogenase activity.