Human Vascular Endothelial Growth Factor cDNA Cloning and Expression in Osteoblasts
摘要
Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD-hVEGF165 recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD-hVEGF165 plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly.
引用本文（GB/T 7714）
Yang, Liping Cao. Human Vascular Endothelial Growth Factor cDNA Cloning and Expression in Osteoblasts[J]. 华中科技大学学报：医学英德文版, 2001.
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