Report of HLA distribution of Chinese Bone Marrow Bank(Shanghai)
摘要
Objective To study the characteristic of HLA distribution of Chinese Bone Marrow Bank(Shanghai),this paper analyses the HLA distribution of a population of 11 995 persons which were major part of this Bank. Methods HLA-A、B antigen of volunteers were detected by MLT(microlymphocytetotoxicity test) method, and at the same time, some samples were selected as control and their AB locus were typed at the DNA level by PCR-SSP method, ambiguous samples were checked by PCR-SSO also.Results HLA-A、B typing results of 11 995 volunteers were gotten, and their antigen frequency, gene frequency, haplotype frequency and linkage disequilibrium parameter were gotten also.Conclusions the HLA-A、B distribution of this population its own characters, according to these characters, we can induce Chines Bone Marrow Bank(Shanghai) were mainly between the south and the north han-population,a little closer to the northern. There are gotten more than 60 binds haplotypes which had significant linkage disequili-brium sense,among them,the most common were A2-B46,A30-B13,A33-B58,A11.1-B60,A24-B62,A24-B60.And the chance of finding out a matched donor from this Bank is roughly one-thousandth.