[Study on HPLC Fingerprint of Solanum nigrum Fruit].
摘要
Objective: To establish the HPLC fingerprint method of Solanum nigrum fruit for its identification and quality evaluation. Methods: The analysis was carried out by Agilent ZORBAX SB-C18column( 150 mm × 4. 6 mm,5 μm),and eluted with mobile phase containing of acetonitrile-0. 3% phosphoric acid in a gradient mode. The temperature of column was 25 ℃,the flow rate was 1. 0m L / min, the detection wavelength was set at 205 nm, the injection volume was 10 μL. The similarity evaluation system for chromatographic fingerprint of TCM( version 2004A) was used to calculate the similarity degree of the HPLC fingerprint of Solanum nigrum fruit. . Results: The HPLC fingerprint of ten batches Solanum nigrum fruit was established with twelve common peaks. Two characteristic peaks included solasonine and solamargine were confirmed. Conclusion: The results indicate that establishing HPLC fingerprint of Solanum nigrum fruit can provide more comprehensive reference for identification and quality evaluation of Solanum nigrum fruit