[Metagenomic Analysis of Wild Rhesus Monkey Virome in Longhu Mountain in Guangxi Area, China].
摘要
Several viruses have been found in non-human primates, among which some are pathogenic to humans. To further characterize the spectrum of viruses present in wild rhesus monkeys, MiSeq high-throughput sequencing were used to analyze 280 fecal samples collected from Guangxi, China. A total of 23,372,679 reads were obtained, of which 4,641 were annotated to 27 viral families or subfamilies, including five vertebrate viruses families(78.2%),six insect virus families(5.5%),eleven plant virus families(10.4%),and other viruses(9.8%).Further analysis revealed that these reads best fit with the sapelovirus, enterovirus, parvovirus, adeno-associated virus read sequences shared a high similarity with the known viruses. However, some reads presented obvious differences from these viruses. Moreover, PCR amplification was conducted to confirm these potentially novel viruses. This study had explored the viral spectrum of rhesus monkey feces in the Guangxi area, which laid the foundation for the potential public health significance of these viruses.