[Anti-CD206 antibody-conjugated Fe<sub>3</sub>O<sub>4</sub>-based PLGA nanoparticles selectively promotes M1 polarization of tumorassociated macrophages in mice].
摘要
OBJECTIVE: To enhance the anti-tumor immunity of macrophages by increasing iron concentration in the macrophages using nanospheres. METHODS: was determined using an iron determination kit. The macrophage-binding and targeting abilities of the conjugated nanoparticles were evaluated using immunofluorescence assay, and the polarization index of macrophages was determined with Western blotting and qRT-PCR. BALB/C-57 mouse models bearing subcutaneous tumors were used to verify the efficacy of the nanoparticles to promote polarization of the tumor-associated macrophages (TAMs). RESULTS: -PLGA nanoparticles were confirmed to promote CD86 expression in the TAMs. CONCLUSIONS: -PLGA nanoparticles are capable of targeted binding to M2 macrophages and reversing the M2 macrophages to M1 phenotype by releasing coated iron oxide particles.