[Time-dependent Expression of Nrf2 Protein and its Effects on Wound Repair in Skeletal Muscle].
摘要
OBJECTIVES: To detect the expression of nuclear factor erythroid 2-related factor 2 （Nrf2） to explore its change rule and effects in the repair process of skeletal muscle. METHODS: An injury model of skeletal muscle was established by injection of cardiotoxin into the gastrocnemius muscle of SD male rats. The muscle samples were taken from control group and injury group at each time point （1 h, 4 h, 8 h, 12 h, 16 h, 1 d, 3 d, 5 d, 7 d, 9 d, 13 d, 17 d, 21 d）. The morphological changes of skeletal muscle in repair process were observed by HE staining, and the content of reactive oxygen species （ROS） was detected. The expression level of Nrf2 protein was investigated by Western blotting and immunohistochemistry staining. RESULTS: <0.05）. CONCLUSIONS: The Nrf2 protein involves in the regulation of the repair of skeletal muscle injury. The positive rate of Nrf2 shows a time-dependent expression, which can be used to estimate the wound age of skeletal muscle.