[Comparison of three different methods for isolating RNA from <i>Oncomelania hupensis</i>].
摘要
OBJECTIVE: . METHODS: . The quality of RNA was inspected by 1% agarose gel electrophoresis. The β-acting gene was selected as the target gene for RT-PCR analysis. RESULTS: ratios of the other two methods were both lower than 2.0. The RNA extracted by the modified SDS method had the better integrity. The electrophoresis results showed that the 28S rRNA band, 18S rRNA band and 5S rRNA band were clear, and there was no obvious smear between each band. The RNA obtained by the TRIzol reagent method had no 28S rRNA band, and that obtained by the CTAB method had no 28S rRNA and 5S rRNA bands. The β-acting gene of the RNA extracted by all the three methods could be amplified by RT-PCR. The costs and time-consuming of the modified SDS method were less than those of the other two methods. CONCLUSIONS: , especially for large sample preparation.