[Effects of Synthetic Long-Chain Polyphosphate on Blood Coagulation and Platelet Aggregation].
摘要
OBJECTIVE: To investigate the effects of synthetic long-chain polyphosphate on blood coagulation and platelet aggregation. METHODS: The effect of artificial synthetic long chain poly phosphate on blood coagulation and platelet aggregation was detected by coagulation tests, coagulation factor activity detection and platelet aggregation test, and its mechanism was explored by ELISA, flow cytometry and high content imaging system. RESULTS: The long chain polyphosphates prolonged activated partial thromboplastin time, decreased coagulation factor FⅧ, FⅨ, FⅪ and FⅫ activity, blocked ADP-induced platelet aggregation, and decreased the concentration of calcium and TXA2 in platelet. CONCLUSION: The synthetic long-chain polyphosphate can inhibit endogenous coagulation and inhibit platelet aggregation, which may be related with the inhibition of intracellular calcium and TXA2.