Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity Partitioning
摘要
The synthesis route was investigated and optimized for the preparation of iminodiacetic acid-polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in aqueous two-phase systems. IDA-PEG was synthesized from PEG in two steps by the reaction of iminodiacetic acid with a monosubstituted derivative of epichlorohydrin-activated PEG. The Cu2+ content combined with IDA-PEG was determined by atomic absorption spectrometry as 0.5 mol·mol^-1 (PEG). Furthermore, the affinity partitioning behavior of lactate dehydrogenase in polyethylene glycol/hydroxypropyl starch aqueous two-phase systems was studied to clarify the affinity effect of the Cu(Ⅱ)-IDA-PEG.
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Dong‐Qiang Lin, 姚善泾, 梅乐和, 等. Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity Partitioning[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2000.
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