牡丹抗逆转录因子基因 PsDREB 的功能解析
摘要
Peony (Paeonia suffruticosa) is one of the most famous traditional flowers with profound cultural connotation in China. With the fast development of garden-flower industry, the application of peony increased dramatically for the purpose of landscaping or medicinal uses. However, stress resistance of peony has been poor due to artificial domestication for thousands of years, which greatly restricted the development of peony. With the significant interest of adding new vitality into the flower industry by successfully introducing and cultivating peony in southern China, improving the tolerance of peony cultivars to stress has become crucial. The dehydration responsive element binding protein (DREB) gene is one of the most important transcription factors that was found to be related to adversity resistance. The objective of this study was to analyze the biological function of DREB gene from peony, named as PsDREB, with methods of yeast one-hybrid assay and transgenic technology.In this study, the ability of PsDREB protein binding with DRE and its transcriptional activation activity were tested by yeast one-hybrid assay.Results showed that the PsDREB protein was able to combine specifically with the DRE, but unable to combine with the mutated DRE (mDRE), showing a strong transcriptional activity. Subsequently, the PsDREB gene was transferred into tobacco and was mediated by Agrobacterium to determine its biological function in response to adversity. Thereafter, the tolerance ability of the transgenic tobacco was detected under manipulated drought, low temperature, high salt, and abscisic acid (ABA) treatments, as compared to controls.In conclusion, the PsDREB protein has strong binding and transcriptional activities. The overexpression of PsDREB can obviously enhance plant resistance to abiotic stresses, especially for drought and high salt treatments. These results lay the foundation for further research on the function mode of PsDREB transcription factor, and also sup