阴离子交换色谱在DNA纳米结构纯化中的应用

Dawei Jiang、Qing Huang、Shu Xing、Lihua Wang、Chengjun Cui

2015Scientia Sinica ChimicaBiochemistry, Genetics and Molecular Biology被引 1

摘要

A purification step after synthesis has become a standard procedure for many DNA nanostructures studies. We firstly applied anion exchange chromatography (AEC) to DNA nanocages purification. A 20 bp-edge DNA bipyramid and five DNA tetrahedra with different edge lengths including 13, 17, 20, 26 and 30 bp were purified effectively by AEC, which improved the purity of these nanostructures from 30%–65% up to 95%. And the yield of a dumbbell-like nanostructure which was constructed using AEC-purified DNA tetrahedra was more than 90%, but 11% for the unpurified group. Given the versatility of this method, AEC is promising for other DNA nanocages and other DNA nanostructures purification.

引用本文（GB/T 7714）

Dawei Jiang, Qing Huang, Shu Xing, 等. 阴离子交换色谱在DNA纳米结构纯化中的应用[J]. Scientia Sinica Chimica, 2015.

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DOI：https://doi.org/10.1360/n032015-00042

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