Hematology of Wild Caught Hoplobatrachus rugulosus in Northern Thailand
摘要
The rice field frog Hoplobatrachus rugulosus is an important anuran species found in wetlands throughout Thailand. At present, the hematological parameters of wild populations are unknown. Therefore, hematological and morphological characteristics of peripheral blood cells of wild-caught H. rugulosus were examined. Thirty-three adult frogs(17 male and 16 female frogs) were collected from a natural population in Nan Province, northern Thailand during the wet season of 2014. Blood samples were analyzed by packed cell volume(PCV) and blood cell counts from hemocytometer and Giemsa-stained blood smears. The mean PCV of male frogs(30.70% ± 6.07%) was significantly higher than that of the female frogs(25.09% ± 4.85%). The mean number of lymphocytes and neutrophils also showed significant sex-related differences. Moreover, the morphometric analysis of blood cells revealed dimensions as follows: erythrocytes(17.96 ± 1.44 μm length × 11.50 ± 1.09 μm width), immature erythrocytes(14.91 ± 2.20 μm diameter), thrombocytes(13.93 ± 3.14 μm length × 7.05 ± 1.31 μm width), lymphocytes(11.01 ± 2.69 μm diameter), monocytes(12.04 ± 2.40 μm diameter), neutrophils(12.58 ± 2.08 μm diameter), basophils(13.60 ± 2.17 μm diameter) and eosinophils(12.33 ± 2.95 μm diameter). Overall, the hematological parameters obtained in this study could be regarded as the first report and a crucial baseline data of wild H.rugulosus in Thailand that can be used for monitoring the health status of this anuran.